Elizabeth C. TheilAdjunct Professor Senior Scientist at Children's Hospital Oakland Research Institute (510) 450-7645 |
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The dietary condition of iron deficiency and genetic diseases, such as sickle cell disease and thalassemia, all have side effects involving ferritin. Molecular properties of ferritin are being studied in solution and cell cultures and with small numbers of human subjects to unravel clinical mysteries and achieve more individualized therapies for sickle cell diseases, thalassemia and iron deficiency. The Theil Molecular BioIron Group in CeBIC (Council for BioIron at CHORI) studies protein, DNA, and mRNA biochemistry and iron nutrition. Click here for more information.
Most Recent Publications
Protein Nanocages
Liu, X. and Theil, E.C. (2005) Ferritins: Dynamic management of biological iron and oxygen chemistry. Acc. Chem. Res. 38: 167-175.
Liu, X., Kim, K., Leighton, T., Theil, E.C. (2006) Paired Bacillus anthracis Dps (mini-ferritin) have different reactivities with peroxide. J. Biol. Chem. 281: 27827-27835.
Matzapetakis, M., Turano, P., Theil, E.C. and Bertini, I. (2007) 13C-13C NOESY spectra of a 480 kDa protein: solution NMR of ferritin. J. Biomol. NMR 38: 237-242.
Bermel, W., Bertini, I., Felli, I.C., Matzapetakis, M., Pierattelli, R., Theil, E.C., Turano, P. (2007) A method for Cα direct-detection in protonless NMR. J. Magn. Reson., 188: 301-310.
Liu, X.S., Patterson, L.D., Miller, M.J. and Theil, E.C. (2007) Peptides selected for the protein nanocage pores change the rate of iron recovery from the ferritin mineral. J. Biol. Chem. 282: 31821-31825.
Schwartz, J.K., Liu, X., Tosha, T., Theil, E.C., Solomon, E.I. (2008) Spectroscopic definition of the ferroxidase site in M ferritin: comparison of binuclear substrate vs. cofactor active sites. J. Am. Chem. Soc.: E-pub: June 25.
Tosha, T., Hasan, M.R., Theil, E.C. (2008) The ferritin Fe2 site at the diiron catalytic center controls the reaction with O2 in the rapid mineralization pathway. Proc. Natl. Acad. Sci. USA 105: 18182-18187.
Hasan, M.R., Tosha, T., Theil, E.C. (2008) Ferritin contains less iron (59Fe) in cells when the protein pores are unfolded by mutation. J. Biol. Chem. 283: 31394-31400.
Ferritin DNA & RNA Regulation
Hintze, K. and Theil, E.C. (2005) DNA and mRNA elements with complementary responses to hemin, antioxidant inducers and iron control ferritin-L expression. Proc. Nat'l. Acad. Sci. USA. 102: 15048-15052.
Hintze K.J., Theil, E.C. (2006) Cellular regulation and molecular interactions of the ferritins. Cell Mol. Life Sci. 63: 591-600
Tibodeau, J.D., Fox, P.M., Ropp, P.A., Theil, E.C., and Thorp, H.H. (2006) The p-regulation of ferritin expression using a small molecule ligand to the native mRNA. Proc. Natl. Acad. Sci. USA 103: 253-257.
Walden, W.E., Selezneva, A.I., Dupuy J., Volbeda, A., Fontecilla-Camps, J.C., Theil, E.C. and Volz, K. (2006) Structure of dual function iron regulatory protein 1 complexed with ferritin IRE-RNA. Science 314: 1903-1908.
Leipuviene, R. and Theil, E.C. (2007) The family of iron responsive RNA structures (IRE) regulated by changes in cellular iron and oxygen. Cell Mol. Life Sci. 64: 2945-2955.
Hintze, K.J., Katoh, Y., Igarashi, K. and Theil, E.C. (2007) Bach1 repression of ferritin and thioredoxin reductase1 is heme-sensitive in cells and in vitro, and coordinates expression with heme oxygenase1, β-globin and NADP(H) quinone (oxido)reductase1. J. Biol. Chem. 282: 34365-34371
Medicine/Nutrition
Theil, E.C. (2004) Iron, ferritin and nutrition. Ann. Rev. Nutr. 24: 327-343. Davila-Hicks, P., Theil, E.C. and Lonnerdal, B.L. (2004) Iron in ferritin or in salts (ferrous sulfate) is equally unavailable in American women. Am. J. Clin. Nutr. 80: 936-940
Lonnerdal, B., Bryant, A., Liu, X., Theil, E.C. (2006) Iron absorption from soybean ferritin in nonanemic women. Am. J. Clin. Nutr. 83: 103-107.
Jenkins, Z.A., Hagar, W., Bowlus, C.L., Johansson, H.E., Harmatz, P., Vichinsky, E.P. and Theil, E.C. (2007) Iron homeostasis during transfusional iron overload in β-thalassemia and sickle cell disease: changes in iron regulatory protein and ferritin expression. Pediatr. Hematol. Oncol. 24: 237-243.
San Martin, C.D., Garri, C., Pizarro, F., Walter, T., Theil, E.C. and Nunez, M.T. (2008) Caco-2 intestinal epithelial cells absorb soybean ferritin by mu2 (AP2)-dependent endocytosis. J. Nutr. 138: 659-666.