Barry Shane
Professor of the Graduate School
Research Description
The major research efforts of my laboratory are in the area of biochemical nutrition and, in particular, the control of one carbon metabolism. We investigate experimental nutrition problems at a basic molecular or cellular level to obtain a better understanding of factors that influence nutritional requirements and how this varies among the population. We study how genetic variation in the pathways of one carbon metabolism influence chronic disease risk and susceptibility to birth defects using humans and genetically modified animal models.
Publications
Carter, T. C., Pangilinan, F., Molloy, A. M., Fan, R., Wang, Y., Shane, B., Gibney, E. R., Midttun, O., Ueland, P. M, Cropp, C. D., Kim, Y., Wilson, A. F., Bailey-Wilson, J. E., Brody, L. C. and Mills, J. L. (2015) Common variants at putative regulatory sites of the tissue nonspecific alkaline phosphatase gene influence circulating pyridoxal 5’-phosphate concentration in healthy adults. J. Nutr. 145: 1386-93.
Deac, O. M., Mills, J. L., Shane, B., Midttun, O., Ueland, P. M., Brosnan, J. T., Brosnan, M. E., Laird, E., Gibney, E. R., Fan, R., Wang, Y., Brody, L. C. and Molloy, A. M. (2015) Tryptophan catabolism and vitamin B-6 status are affected by gender and lifestyle factors in healthy young adults. J. Nutr. 145: 701-7.
Ozaki, M., Molloy, A. M., Mills, J. L., Fan, R., Wang, Y., Gibney, E. R., Shane, B., Brody, L. C. and Parle-McDermott, A. (2015) The Dihydrofolate Reductase 19bp polymorphism has no significant impact on biomarkers of folate status in healthy young adults, irrespective of folic acid intake. J. Nutr. 145: 2207-11.
Molloy, A. M., Pangilinan, F., Mills, J. L., Shane, B., O’Neill, M. B., McGaughey, D. M., Velkova, A., Abaan, H. O., Ueland, P. M., McNulty, H., Ward, M., Strain, J. J., Cunningham, C., Casey, M., Cropp, C. D., Kim, Y., Bailey-Wilson, J. E., Wilson, A. F. and Brody, L. C. (2016) A common polymorphism in HIBCH influences methylmalonic acid concentrations in blood independently of cobalamin. Am. J. Hum. Genet. 98: 869–882.
Deac, O. M., Mills, J. L., Gardiner, C. M., Shane, B., Quinn, L., Midttun, Ø., McCann, A., Meyer, K., Ueland, P. M., Fan, R., Lu, Z., Brody, L. C. and Molloy, A. M. (2016) Serum immune system biomarkers neopterin and interleukin-10 are strongly related to tryptophan metabolism in healthy young adults. J. Nutr. 146: 1801-1806 doi: 10.3945/jn.116.230698
Velkova, A., Diaz, J. E. L., Pangilinan, F., Molloy, A. M., Mills, J. L., Shane, B., Sanchez, E., Cunningham, C., McNulty, H., Cropp, C. D., Bailey-Wilson, J. E., Wilson, A. F. and Brody, L. C. (2017) The FUT2 secretor variant p.Trp154Ter influences serum vitamin B12 concentration via holo-haptocorrin, but not holo-transcobalamin, and is associated with haptocorrin glycosylation. Hum. Mol. Genet. 26: 4975-4988.
Brosnan, J. T., Mills, J. L., Ueland, P. M., Shane, B., Fan, R., Chiu, C.-Y., Pangilinan, F., Brody, L. C., Brosnan, M. E., Pongnopparat, T. and Molloy, A. M. (2018) Lifestyle, metabolite, and genetic determinants of formate concentrations in a cross-sectional study in young, healthy adults. Am. J. Clin. Nutr. 107: 345-354.
Shane, B., Pangilinan, F., Mills, J. L., Fan, R., Gong, T., Cropp, C. D., Kim, Y., Ueland, P. M., Bailey-Wilson, J. E., Wilson, A. F., Brody, L. C. and Molloy, A. M. (2018) The 677C->T variant of MTHFR is the major genetic modifier of biomarkers of folate status in a young healthy Irish population. Am. J. Clin. Nutr. 108: 1334-1341.
Wang, Y-C., Wu, M-T., Tang, F-Y., Chen, D-Y., Ko, H-A., Shane, B., Huang, W-N. and Chieng, E-P. (2019). MTHFR C677T Polymorphism Increases Methotrexate Sensitivity via the inhibition of S-adenosylmethionine and De Novo Purine Synthesis. Clin. Sci. 133: 253-267.
Maruvada P., Patrick J Stover, P. J., Mason, J. B., Bailey, R. L., Davis, C. D., Field, M., Finnell, R. H., Garza, C., Green, R., Gueant, J.-L., Jacques, P. F., Johnston, B., Klurfeld, D. M., Lamers, Y., MacFarlane, A., Miller, J. W., Molloy, A. M., O’Connor, D. L., Pfeiffer, C. M., Potischman, N. A., Rodricks, J. V., Rosenberg, I. H., Shane, B., Selhub, J., Ross, S. A., Stabler, S. P., Trasler, J., Yamini, S. and Zappala, G. (2020). Knowledge gaps in understanding the metabolic and clinical effects of excess folates/folic acid: a summary, and perspectives, from an NIH workshop. Am. J. Clin. Nutr. 112: 1390-1403.
Pangilinan, F., Finlay E. K., Molloy, A. M., Abaan, H. O., Shane, B., Mills J. L., Brody, L. C. and Parle-McDermott, A. (2021). A Dihydrofolate Reductase 2 (DHFR2) Variant is Associated with Risk of Neural Tube Defects in an Irish Cohort but not in a United Kingdom Cohort. Amer J Med Gen Part A 2021;1-5. DOI: 10.1002/ajmg.a.62090
Sou, N. L., Huang, Y. H, Chen, D. Y., Chen, Y. M., Tang, F. Y., Ko, H. A., Fan, Y. H., Lin, Y. Y., Wang, Y. C., Chih, H. M., Shane, B., Huang, W. N. and Chiang, E. P. (2021). Folinate supplementation ameliorates methotrexate induced mitochondrial formate depletion in vitro and in vivo. Int. J. Molec. Sci. 22: 1350. doi.org/10.3390/ijms22
