Andreas Stahl

Professor

Research Description

The Stahl Lab

The overarching goal of our laboratory is to advance human health by re-engineer lipid fluxes in the context of obesity-related disorders to protect certain tissues, such as the liver, from detrimental effects of ectopic lipid deposition and to generate metabolically highly active tissues that can serve as a save destination for excess fatty acids. To this end, we are investigating molecular mechanisms governing lipid uptake, particularly for fatty acids and CoQ, hepatobiliary diseases, liver cancer, and adipocyte biology. Toward the latter, we have been working on novel tissue-engineering based approaches to expand and activate brown adipose fat. Further, to facilitate the assessment of altered nutrient fluxes, we have been developing and testing novel bioluminescent imaging approaches to quantitatively assess macro- and micronutrients in vivo and have been working as part of a multidisciplinary team toward modeling human metabolic function and disease using iPSC-derived microphysiological devices, a.k.a. organs-on-a-chip, for adipocytes, hepatocytes, and islets.

Adipose Tissue Biology

We have a longstanding interest in brown adipose tissue (BAT) biology, determination of fatty acid uptake rates for this tissue, and its application in anti-obesity approaches. Toward the latter, we have pursued tissue-engineering approaches to create stem cell-derived brown fat implants that also have shed light on unexpected roles for the cellular tension generation and sensing in BAT activation.

A major recent effort was to establish a role for actomyosin-mediated tension in BAT activation. This work, in collaboration with Dr. Kumar in bioengineering, led to a completely novel paradigm for biomechanical signaling in BAT connecting muscle like contraction with nuclear translocation of the transcriptional activators YAP/TAZ and induction of uncoupled respiration.

Another area we continue to pursue is the expansion of BAT via implantation strategies. Our overall hypothesis is that bioinspired hyaluronic acid hydrogels can be engineered to drive and support differentiation of white adipose tissue derived mesenchymal stem cells (ADMSC) into functional BAT that can be used for autologous implantation to enhance metabolic rates and to promote weight loss and insulin sensitivity in obese subjects. To this end we have developed BAT optimized hydrogels to support the differentiation of ADMSCs into BAT and the maintenance of functional BAT matrix assisted cell transplants in vivo.

Fatty Acid/Lipid Transport

My laboratory, first at the Palo Alto Medical Foundation/Stanford University and now at UC Berkeley, has made seminal contributions to protein mediated fatty acid uptake and metabolic biology. We were the first to identify an intestinal fatty acid transporter, to demonstrate the existence of an insulin-sensitive fatty acid transporter in adipocytes, and to identify a cardiac specific FATP. We demonstrated a role of FATPs in hepatobiliary diseases including gallstone disease and hepatic carcinomas.

Recently, we were able to show that gallbladder hypomotility, a major contributor to gallstone disease, results from FATP2-mediated fatty acid uptake by gallbladder epithelial cells, and that deletion of FATP2 protects animals from diet-induced gallstone formation.

Another exciting research area for the role of lipid uptake in human diseases is liver cancer. In a collaborative effort with researchers at UCSF we are investigating the growth dependence of hepatocellular- and cholangiocarcinomas on the protein mediated uptake of exogenous fatty acids.

We have recently expanded on the theme of lipid transport by uncovering novel transport mechanisms for CoQ in BAT. We were the first to describe a function for CD36 in brown adipose tissue CoQ uptake and have now created knockout models to create CoQ synthesis defects in BAT. We are using these complementary systems to investigate how CoQ deficiency regulates thermogenic gene expression and BAT function, the physiological regulation of CoQ pools in BAT, and the ability of dietary CoQ to overcome CoQ deficiencies in mitochondrial function and thermogenesis.

Novel Imaging Probes for Nutrient Fluxes

In order to visualize changes in organismal lipid fluxes we have developed novel imaging probes in conjunction with researchers in the Chemistry Department. We showed that a bioluminescent fatty acid probe can be utilized in live animals for the quantitative spatio-temporally resolved detection of cellular fatty acid fluxes. We are expanding on this collaboration by developing and testing additional probes for the bioluminescent detection of macro- and micro-nutrients in vivo.

Recently our lab provided a major technical advance to the field of Hepatology by introducing a system for the in vivo imaging of hepatic fatty acids. Using this system, we were for example able to detect the action of fatty acid uptake inhibitors in vivo as well as the diurnal rhythm of liver fatty acid absorption. We also have continued our close collaboration with Dr. Chang in chemistry and utilized a novel copper probe to demonstrate copper deficiency during the development of hepatosteatosis.

Micorphysiological Systems to Model Human Metabolic Networks

To complement animal models of human diseases and to provide insights into physiological and pathological interactions between human tissues, we are also developing and testing “organ-on-a-chip” devices for metabolic studies.

We are spearheading interdisciplinary efforts involving multiple groups both at UCB and UCSF to create human iPSC-derived “organ-on-a-chip” mirophysiologycal devices for drug development and metabolic studies. To expedite the search for novel obesity and diabetes treatments, we are developing an essential set of human stem cell-derived metabolic tissues for glucose and fatty acid uptake/release (liver and adipose) and insulin secretion (islets) in conjunction with an immune component (macrophages) using interconnected microphysiological systems (MPS). Combining liver-, adipose- and islet-MPS will allow the pharmacological interrogation of glucose and insulin sensitivity in the context of normal tissue interactions, lipid overload and chronic inflammation.

Publications

Publications

1.         Buss E, Stahl A, Geertz R, Gercken G. Cytotoxic effects of dust particles on alveolar macrophages. Journal of Aerosol Science. 1990;21, Supplement 1(0):S497-S500.

2.         Stahl A, Mueller BM. Binding of urokinase to its receptor promotes migration and invasion of human melanoma cells in vitro. Cancer research. 1994;54(11):3066-71. PubMed PMID: 8187097.

3.         Stahl A, Mueller BM. The urokinase-type plasminogen activator receptor, a GPI-linked protein, is localized in caveolae. J Cell Biol. 1995;129(2):335-44. PubMed PMID: 7721938; PMCID: 2199914.

4.         Stahl A, Mueller BM. Melanoma cell migration on vitronectin: regulation by components of the plasminogen activation system. International journal of cancer. 1997;71(1):116-22. PubMed PMID: 9096674.

5.         Hirsch D, Stahl A, Lodish HF. A family of fatty acid transporters conserved from mycobacterium to man. Proc Natl Acad Sci U S A. 1998;95(15):8625-9.

6.         Stahl A, Hirsch DJ, Gimeno RE, Punreddy S, Ge P, Watson N, Patel S, Kotler M, Raimondi A, Tartaglia LA, Lodish HF. Identification of the major intestinal fatty acid transport protein. Mol Cell. 1999;4(3):299-308. PubMed PMID: 10518211.

7.         Stahl A, Gimeno RE, Tartaglia LA, Lodish HF. Fatty acid transport proteins: a current view of a growing family. Trends in endocrinology and metabolism: TEM. 2001;12(6):266-73.

8.         Stahl A, Evans JG, Pattel S, Hirsch D, Lodish HF. Insulin causes fatty acid transport protein translocation and enhanced fatty acid uptake in adipocytes. Dev Cell. 2002;2(4):477-88.

9.         Gimeno RE, Hirsch DJ, Punreddy S, Sun Y, Ortegon AM, Wu H, Daniels T, Stricker-Krongrad A, Lodish HF, Stahl A. Targeted deletion of fatty acid transport protein-4 results in early embryonic lethality. J Biol Chem. 2003;278(49):49512-6. PubMed PMID: 14512415.

10.       Gimeno RE, Ortegon AM, Patel S, Punreddy S, Ge P, Sun Y, Lodish HF, Stahl A. Characterization of a heart-specific fatty acid transport protein. J Biol Chem. 2003;278(18):16039-44.

11.       Kim JK, Gimeno RE, Higashimori T, Kim HJ, Choi H, Punreddy S, Mozell RL, Tan G, Stricker-Krongrad A, Hirsch DJ, Fillmore JJ, Liu ZX, Dong J, Cline G, Stahl A, Lodish HF, Shulman GI. Inactivation of fatty acid transport protein 1 prevents fat-induced insulin resistance in skeletal muscle. J Clin Invest. 2004;113(5):756-63. PubMed PMID: 14991074.

12.       Mashek DG, Bornfeldt KE, Coleman RA, Berger J, Bernlohr DA, Black P, DiRusso CC, Farber SA, Guo W, Hashimoto N, Khodiyar V, Kuypers FA, Maltais LJ, Nebert DW, Renieri A, Schaffer JE, Stahl A, Watkins PA, Vasiliou V, Yamamoto TT. Revised nomenclature for the long chain mammalian acyl-CoA synthetase gene family. J Lipid Res. 2004. PubMed PMID: 15292367.

13.       Stahl A. A current review of fatty acid transport proteins (SLC27). Pflugers Arch. 2004;447(5):722-7. PubMed PMID: 12856180.

14.       Stiles B, Wang Y, Stahl A, Bassilian S, Lee WP, Kim YJ, Sherwin R, Devaskar S, Lesche R, Magnuson MA, Wu H. Live-specific deletion of negative regulator Pten results in fatty liver and insulin hypersensitivity. Proc Natl Acad Sci U S A. 2004;101(7):2082-7. PubMed PMID: 14769918.

15.       Yu KC, David C, Kadambi S, Stahl A, Hirata K, Ishida T, Quertermous T, Cooper AD, Choi SY. Endothelial lipase is synthesized by hepatic and aorta endothelial cells and its expression is altered in apoE-deficient mice. J Lipid Res. 2004;45(9):1614-23. PubMed PMID: 15175355.

16.       Johnson AC, Stahl A, Zager RA. Triglyceride accumulation in injured renal tubular cells: alterations in both synthetic and catabolic pathways. Kidney Int. 2005;67(6):2196-209. PubMed PMID: 15882263.

17.       Liao J, Sportsman R, Harris J, Stahl A. Real-time quantification of fatty acid uptake using a novel fluorescence assay. J Lipid Res. 2005;46(3):597-602. PubMed PMID: 15547301.

18.       Schmuth M, Ortegon AM, Mao-Qiang M, Elias PM, Feingold KR, Stahl A. Differential expression of fatty acid transport proteins in epidermis and skin appendages. J Invest Dermatol. 2005;125(6):1174-81. Epub 2005/12/16. doi: 10.1111/j.0022-202X.2005.23934.x. PubMed PMID: 16354187.

19.       Stahl A. A case of mistaken identity. Am J Physiol Heart Circ Physiol. 2005;288(1):H448; author reply H-50. PubMed PMID: 15598873.

20.       Doege H, Baillie RA, Ortegon AM, Tsang B, Wu Q, Punreddy S, Hirsch D, Watson N, Gimeno RE, Stahl A. Targeted deletion of FATP5 reveals multiple functions in liver metabolism: alterations in hepatic lipid homeostasis. Gastroenterology. 2006;130(4):1245-58. PubMed PMID: 16618416.

21.       Doege H, Stahl A. Protein-mediated fatty acid uptake: novel insights from in vivo models. Physiology (Bethesda). 2006;21:259-68. PubMed PMID: 16868315.

22.       Hubbard B, Doege H, Punreddy S, Wu H, Huang X, Kaushik VK, Mozell RL, Byrnes JJ, Stricker-Krongrad A, Chou CJ, Tartaglia LA, Lodish HF, Stahl A, Gimeno RE. Mice deleted for fatty acid transport protein 5 have defective bile acid conjugation and are protected from obesity. Gastroenterology. 2006;130(4):1259-69. PubMed PMID: 16618417.

23.       Wu Q, Kazantzis M, Doege H, Ortegon AM, Tsang B, Falcon A, Stahl A. Fatty acid transport protein 1 is required for nonshivering thermogenesis in brown adipose tissue. Diabetes. 2006;55(12):3229-37. Epub 2006/11/30. doi: 55/12/3229 [pii]

10.2337/db06-0749. PubMed PMID: 17130465.

24.       Wu Q, Ortegon AM, Tsang B, Doege H, Feingold KR, Stahl A. FATP1 is an insulin-sensitive fatty acid transporter involved in diet-induced obesity. Mol Cell Biol. 2006;26(9):3455-67. PubMed PMID: 16611988.

25.       Doege H, Grimm D, Falcon A, Tsang B, Storm TA, Xu H, Ortegon AM, Kazantzis M, Kay MA, Stahl A. Silencing of hepatic fatty acid transporter protein 5 in vivo reverses diet-induced non-alcoholic fatty liver disease and improves hyperglycemia. J Biol Chem. 2008;283(32):22186-92. Epub 2008/06/06. doi: M803510200 [pii]

10.1074/jbc.M803510200. PubMed PMID: 18524776.

26.       Doege H, Stahl A. Fatty Acid Transporters. In: Rosenthal SOaW, editor. Encyclopedia of Molecular Pharmacology. Berlin: Springer; 2008. p. 494-8.

27.       Falcon A, Doege H, Fluitt A, Tsang B, Watson N, Kay MA, Stahl A. FATP2 is a hepatic fatty acid transporter and peroxisomal very long-chain acyl-CoA synthetase. Am J Physiol Endocrinol Metab. 2010. Epub 2010/06/10. doi: ajpendo.00226.2010 [pii]

10.1152/ajpendo.00226.2010 [doi]. PubMed PMID: 20530735.

28.       Ryu KY, Fujiki N, Kazantzis M, Garza JC, Bouley DM, Stahl A, Lu XY, Nishino S, Kopito RR. Loss of polyubiquitin gene Ubb leads to metabolic and sleep abnormalities in mice. Neuropathology and applied neurobiology. 2010;36(4):285-99. doi: 10.1111/j.1365-2990.2009.01057.x. PubMed PMID: 20002312; PMCID: 3029017.

29.       Zhou W, Madrid P, Fluitt A, Stahl A, Xie XS. Development and validation of a high-throughput screening assay for human long-chain fatty acid transport proteins 4 and 5. J Biomol Screen. 2010;15(5):488-97. Epub 2010/05/08. doi: 1087057110369700 [pii]

10.1177/1087057110369700. PubMed PMID: 20448275; PMCID: 2887688.

30.       Kazantzis M, Stahl A. Fatty acid transport proteins, implications in physiology and disease. Biochim Biophys Acta. 2011. Epub 2011/10/08. doi: 10.1016/j.bbalip.2011.09.010. PubMed PMID: 21979150.

31.       Mishima T, Miner JH, Morizane M, Stahl A, Sadovsky Y. The expression and function of Fatty Acid transport protein-2 and -4 in the murine placenta. PLoS One. 2011;6(10):e25865. Epub 2011/10/27. doi: 10.1371/journal.pone.0025865. PubMed PMID: 22028793; PMCID: 3197585.

32.       Abbasi A, Thamotharan M, Shin BC, Jordan MC, Roos KP, Stahl A, Devaskar SU. Myocardial macronutrient transporter adaptations in the adult pregestational female intrauterine and postnatal growth-restricted offspring. Am J Physiol Endocrinol Metab. 2012;302(11):E1352-62. doi: 10.1152/ajpendo.00539.2011. PubMed PMID: 22338075; PMCID: 3378069.

33.       Henkin AH, Cohen AS, Dubikovskaya EA, Park HM, Nikitin GF, Auzias MG, Kazantzis M, Bertozzi CR, Stahl A. Real-time noninvasive imaging of fatty acid uptake in vivo. ACS Chem Biol. 2012;7(11):1884-91. Epub 2012/08/30. doi: 10.1021/cb300194b. PubMed PMID: 22928772; PMCID: 3500440.

34.       Henkin AH, Ortegon AM, Cho S, Shen WJ, Falcon A, Kraemer FB, Lee SJ, Stahl A. Evidence for protein-mediated fatty acid efflux by adipocytes. Acta Physiol (Oxf). 2012;204(4):562-70. Epub 2011/09/29. doi: 10.1111/j.1748-1716.2011.02367.x. PubMed PMID: 21951599; PMCID: 3271185.

35.       Jeppesen J, Jordy AB, Sjoberg KA, Fullekrug J, Stahl A, Nybo L, Kiens B. Enhanced Fatty Acid Oxidation and FATP4 Protein Expression after Endurance Exercise Training in Human Skeletal Muscle. PLoS One. 2012;7(1):e29391. Epub 2012/01/12. doi: 10.1371/journal.pone.0029391. PubMed PMID: 22235293.

36.       Nie B, Park HM, Kazantzis M, Lin M, Henkin A, Ng S, Song S, Chen Y, Tran H, Lai R, Her C, Maher JJ, Forman BM, Stahl A. Specific bile acids inhibit hepatic fatty acid uptake in mice. Hepatology. 2012;56(4):1300-10. doi: 10.1002/hep.25797. PubMed PMID: 22531947; PMCID: 3445775.

37.       Poreba MA, Dong CX, Li SK, Stahl A, Miner JH, Brubaker PL. Role of fatty acid transport protein 4 in oleic acid-induced glucagon-like peptide-1 secretion from murine intestinal L cells. Am J Physiol Endocrinol Metab. 2012;303(7):E899-907. doi: 10.1152/ajpendo.00116.2012. PubMed PMID: 22871340; PMCID: 3469616.

38.       Anderson CM, Stahl A. SLC27 fatty acid transport proteins. Molecular aspects of medicine. 2013;34(2-3):516-28. doi: 10.1016/j.mam.2012.07.010. PubMed PMID: 23506886; PMCID: 3602789.

39.       Godinat A, Park HM, Miller SC, Cheng K, Hanahan D, Sanman LE, Bogyo M, Yu A, Nikitin GF, Stahl A, Dubikovskaya EA. A Biocompatible in Vivo Ligation Reaction and Its Application for Noninvasive Bioluminescent Imaging of Protease Activity in Living Mice. ACS Chem Biol. 2013. Epub 2013/03/08. doi: 10.1021/cb3007314. PubMed PMID: 23463944.

40.       Khalifeh-Soltani A, McKleroy W, Sakuma S, Cheung YY, Tharp K, Qiu Y, Turner SM, Chawla A, Stahl A, Atabai K. Mfge8 promotes obesity by mediating the uptake of dietary fats and serum fatty acids. Nature medicine. 2014. Epub 2014/01/21. doi: 10.1038/nm.3450. PubMed PMID: 24441829.

41.       Dubikovskaya E, Chudnovskiy R, Karateev G, Park HM, Stahl A. Measurement of long-chain fatty acid uptake into adipocytes. Methods in enzymology. 2014;538:107-34. Epub 2014/02/18. doi: 10.1016/b978-0-12-800280-3.00007-4. PubMed PMID: 24529436.

42.       Godinat A, Budin G, Morales AR, Park HM, Sanman LE, Bogyo M, Yu A, Stahl A, Dubikovskaya EA. A biocompatible "split luciferin" reaction and its application for non-invasive bioluminescent imaging of protease activity in living animals. Current protocols in chemical biology. 2014;6(3):169-89. Epub 2014/09/11. doi: 10.1002/9780470559277.ch140047. PubMed PMID: 25205565.

43.       Ye R, Holland WL, Gordillo R, Wang M, Wang QA, Shao M, Morley TS, Gupta RK, Stahl A, Scherer PE. Adiponectin is essential for lipid homeostasis and survival under insulin deficiency and promotes beta-cell regeneration. eLife. 2014;3. Epub 2014/10/24. doi: 10.7554/eLife.03851. PubMed PMID: 25339419.

44.       Chudnovskiy R, Thompson A, Tharp K, Hellerstein M, Napoli JL, Stahl A. Consumption of clarified grapefruit juice ameliorates high-fat diet induced insulin resistance and weight gain in mice. PLoS One. 2014;9(10):e108408. doi: 10.1371/journal.pone.0108408. PubMed PMID: 25296035; PMCID: 4189915.

45.       Anderson CM, Kazantzis M, Wang J, Venkatraman S, Goncalves RL, Quinlan CL, Ng R, Jastroch M, Benjamin DI, Nie B, Herber C, Van AA, Park MJ, Yun D, Chan K, Yu A, Vuong P, Febbraio M, Nomura DK, Napoli JL, Brand MD, Stahl A. Dependence of Brown Adipose Tissue Function on CD36-Mediated Coenzyme Q Uptake. Cell reports. 2015. doi: 10.1016/j.celrep.2014.12.048. PubMed PMID: 25620701.

46.       Jha AK, Tharp KM, Ye J, Santiago-Ortiz JL, Jackson WM, Stahl A, Schaffer DV, Yeghiazarians Y, Healy KE. Enhanced survival and engraftment of transplanted stem cells using growth factor sequestering hydrogels. Biomaterials. 2015;47(0):1-12. doi: http://dx.doi.org/10.1016/j.biomaterials.2014.12.043.

47.       Tharp KM, Stahl A. Bioengineering Beige Adipose Tissue Therapeutics. Frontiers in endocrinology. 2015;6:164. doi: 10.3389/fendo.2015.00164. PubMed PMID: 26539163; PMCID: PMC4611961.

48.       Tharp KM, Jha AK, Kraiczy J, Yesian A, Karateev G, Sinisi R, Dubikovskaya EA, Healy KE, Stahl A. Matrix-Assisted Transplantation of Functional Beige Adipose Tissue. Diabetes. 2015;64(11):3713-24. doi: 10.2337/db15-0728. PubMed PMID: 26293504; PMCID: PMC4613967.

49.       Li L, Che L, Tharp KM, Park HM, Pilo MG, Cao D, Cigliano A, Latte G, Xu Z, Ribback S, Dombrowski F, Evert M, Gores GJ, Stahl A, Calvisi DF, Chen X. Differential Requirement for de novo Lipogenesis in Cholangiocarcinoma and Hepatocellular Carcinoma of Mice and Humans. Hepatology. 2016. Epub 2016/02/26. doi: 10.1002/hep.28508. PubMed PMID: 26910791.

50.       Jha AK, Tharp KM, Browne S, Ye J, Stahl A, Yeghiazarians Y, Healy KE. Matrix metalloproteinase-13 mediated degradation of hyaluronic acid-based matrices orchestrates stem cell engraftment through vascular integration. Biomaterials. 2016;89:136-47. Epub 2016/03/12. doi: 10.1016/j.biomaterials.2016.02.023. PubMed PMID: 26967648; PMCID: PMC4851169.

51.       Tharp KM, Khalifeh-Soltani A, Park HM, Yurek DA, Falcon A, Wong L, Feng R, Atabai K, Stahl A. Prevention of gallbladder hypomotility via FATP2 inhibition protects from lithogenic diet-induced cholelithiasis. American Journal of Physiology - Gastrointestinal and Liver Physiology. 2016:ajpgi.00316.2015. doi: 10.1152/ajpgi.00316.2015.

52.       Khalifeh-Soltani A, Ha A, Podolsky MJ, McCarthy DA, McKleroy W, Azary S, Sakuma S, Tharp KM, Wu N, Yokosaki Y, Hart DO, Stahl A, Atabai K. alpha8beta1 integrin regulates nutrient absorption through an Mfge8-PTEN dependent mechanism. eLife. 2016;5. Epub 2016/04/20. doi: 10.7554/eLife.13063. PubMed PMID: 27092791.

53.       Johnson AR, Qin Y, Cozzo AJ, Freemerman AJ, Huang MJ, Zhao L, Sampey BP, Milner JJ, Beck MA, Damania B, Rashid N, Galanko JA, Lee DP, Edin ML, Zeldin DC, Fueger PT, Dietz B, Stahl A, Wu Y, Mohlke KL, Makowski L. Metabolic reprogramming through fatty acid transport protein 1 (FATP1) regulates macrophage inflammatory potential and adipose inflammation. Molecular metabolism. 2016. doi: 10.1016/j.molmet.2016.04.005.

54.       Sambeat A, Gulyaeva O, Dempersmier J, Tharp KM, Stahl A, Paul SM, Sul HS. LSD1 Interacts with Zfp516 to Promote UCP1 Transcription and Brown Fat Program. Cell reports. 2016;15(11):2536-49. Epub 2016/06/07. doi: 10.1016/j.celrep.2016.05.019. PubMed PMID: 27264172.

55.       Park HM, Russo KA, Karateev G, Park M, Dubikovskaya E, Kriegsfeld LJ, Stahl A. A System for In Vivo Imaging of Hepatic Free Fatty Acid Uptake. Gastroenterology. 2016. Epub 2016/10/16. doi: 10.1053/j.gastro.2016.10.002. PubMed PMID: 27742378.

56.       Secco B, Camire E, Briere MA, Caron A, Billong A, Gelinas Y, Lemay AM, Tharp KM, Lee PL, Gobeil S, Guimond JV, Patey N, Guertin DA, Stahl A, Haddad E, Marsolais D, Bosse Y, Birsoy K, Laplante M. Amplification of Adipogenic Commitment by VSTM2A. Cell reports. 2017;18(1):93-106. Epub 2017/01/05. doi: 10.1016/j.celrep.2016.12.015. PubMed PMID: 28052263.

57.       Heffern MC, Park HM, Au-Yeung HY, Van de Bittner GC, Ackerman CM, Stahl A, Chang CJ. In vivo bioluminescence imaging reveals copper deficiency in a murine model of nonalcoholic fatty liver disease. Proc Natl Acad Sci U S A. 2016;113(50):14219-24. doi: 10.1073/pnas.1613628113. PubMed PMID: 27911810; PMCID: PMC5167165.

58.       Loskill P, Sezhian T, Tharp K, Lee-Montiel FT, Jeeawoody S, Reese WM, Zushin P-JH, Stahl A, Healy K. WAT-on-a-chip: A physiologically relevant microfluidic system incorporating white adipose tissue. Lab on a Chip. 2017. doi: 10.1039/C6LC01590E.

59.       Riera CE, Tsaousidou E, Halloran J, Follett P, Hahn O, Pereira MMA, Ruud LE, Alber J, Tharp K, Anderson CM, Bronneke H, Hampel B, Filho CDM, Stahl A, Bruning JC, Dillin A. The Sense of Smell Impacts Metabolic Health and Obesity. Cell Metab. 2017;26(1):198-211 e5. doi: 10.1016/j.cmet.2017.06.015. PubMed PMID: 28683287.

60.       Aron AT, Heffern MC, Lonergan ZR, Vander Wal MN, Blank BR, Spangler B, Zhang Y, Park HM, Stahl A, Renslo AR, Skaar EP, Chang CJ. In vivo bioluminescence imaging of labile iron accumulation in a murine model of Acinetobacter baumannii infection. Proc Natl Acad Sci U S A. 2017;114(48):12669-74. Epub 2017/11/16. doi: 10.1073/pnas.1708747114. PubMed PMID: 29138321; PMCID: PMC5715752.

61.       Tharp KM, Kang MS, Timblin GA, Dempersmier J, Dempsey GE, Zushin PH, Benavides J, Choi C, Li CX, Jha AK, Kajimura S, Healy KE, Sul HS, Saijo K, Kumar S, Stahl A. Actomyosin-Mediated Tension Orchestrates Uncoupled Respiration in Adipose Tissues. Cell Metab. 2018;27(3):602-15 e4. Epub 2018/03/08. doi: 10.1016/j.cmet.2018.02.005. PubMed PMID: 29514068.

62.       Miranda DA, Krause WC, Cazenave-Gassiot A, Suzawa M, Escusa H, Foo JC, Shihadih DS, Stahl A, Fitch M, Nyangau E, Hellerstein M, Wenk MR, Silver DL, Ingraham HA. LRH-1 regulates hepatic lipid homeostasis and maintains arachidonoyl phospholipid pools critical for phospholipid diversity. JCI Insight. 2018;3(5). Epub 2018/03/09. doi: 10.1172/jci.insight.96151. PubMed PMID: 29515023.

63.       West MD, Chang CF, Larocca D, Li J, Jiang J, Sim P, Labat I, Chapman KB, Wong KE, Nicoll J, Van Kanegan MJ, de Grey A, Nasonkin IO, Stahl A, Sternberg H. Clonal derivation of white and brown adipocyte progenitor cell lines from human pluripotent stem cells. Stem cell research & therapy. 2019;10(1):7. Epub 2019/01/09. doi: 10.1186/s13287-018-1087-7. PubMed PMID: 30616682; PMCID: PMC6323697.

64.       Su TA, Shihadih DS, Cao W, Detomasi TC, Heffern MC, Jia S, Stahl A, Chang CJ. A Modular Ionophore Platform for Liver-Directed Copper Supplementation in Cells and Animals. J Am Chem Soc. 2018;140(42):13764-74. Epub 2018/10/24. doi: 10.1021/jacs.8b08014. PubMed PMID: 30351140; PMCID: PMC6465169.

65.       Hughes JH, Ewy JM, Chen J, Wong SY, Tharp KM, Stahl A, Kumar S. Transcriptomic analysis reveals that BMP4 sensitizes glioblastoma tumor-initiating cells to mechanical cues. Matrix Biol. 2019. Epub 2019/06/13. doi: 10.1016/j.matbio.2019.06.002. PubMed PMID: 31189077.

66.       Castriota F, Zushin PH, Sanchez SS, Phillips RV, Hubbard A, Stahl A, Smith MT, Wang JC, La Merrill MA. Chronic arsenic exposure impairs adaptive thermogenesis in male C57BL/6J mice. Am J Physiol Endocrinol Metab. 2020;318(5):E667-e77. Epub 2020/02/12. doi: 10.1152/ajpendo.00282.2019. PubMed PMID: 32045263; PMCID: PMC7272725.

67.       Zhang Y, Shen WJ, Qiu S, Yang P, Dempsey G, Zhao L, Zhou Q, Hao X, Dong D, Stahl A, Kraemer FB, Leung LL, Morser J. Chemerin regulates formation and function of brown adipose tissue: Ablation results in increased insulin resistance with high fat challenge and aging. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2021;35(7):e21687. Epub 2021/06/06. doi: 10.1096/fj.202100156R. PubMed PMID: 34089273.

68.       Timblin GA, Tharp KM, Ford B, Winchester JM, Wang J, Zhu S, Khan RI, Louie SK, Iavarone AT, Ten Hoeve J, Nomura DK, Stahl A, Saijo K. Mitohormesis reprogrammes macrophage metabolism to enforce tolerance. Nat Metab. 2021;3(5):618-35. Epub 2021/05/26. doi: 10.1038/s42255-021-00392-w. PubMed PMID: 34031590; PMCID: PMC8162914.

69.       Lei R, Akins EA, Wong KCY, Repina NA, Wolf KJ, Dempsey GE, Schaffer DV, Stahl A, Kumar S. Multiwell Combinatorial Hydrogel Array for High-Throughput Analysis of Cell-ECM Interactions. ACS Biomater Sci Eng. 2021. Epub 2021/05/25. doi: 10.1021/acsbiomaterials.1c00065. PubMed PMID: 34028263.

70.       Moldavski O, Zushin PH, Berdan CA, Van Eijkeren RJ, Jiang X, Qian M, Ory DS, Covey DF, Nomura DK, Stahl A, Weiss EJ, Zoncu R. 4β-Hydroxycholesterol is a prolipogenic factor that promotes SREBP1c expression and activity through the liver X receptor. J Lipid Res. 2021;62:100051. Epub 2021/02/26. doi: 10.1016/j.jlr.2021.100051. PubMed PMID: 33631213; PMCID: PMC8042401.